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Enzyme-catalyzed expressed protein ligation
Expressed protein ligation is a valuable method for protein semisynthesis that involves the reaction of recombinant protein C-terminal thioesters with N-Cys containing peptides but the requirement of a Cys residue at the ligation junction can limit its use. Here we employ subtiligase variants to eff...
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| 出版年: | Nat Methods |
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| 主要な著者: | , , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2016
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5088058/ https://ncbi.nlm.nih.gov/pubmed/27669326 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4004 |
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