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Enzyme-catalyzed expressed protein ligation
Expressed protein ligation is a valuable method for protein semisynthesis that involves the reaction of recombinant protein C-terminal thioesters with N-Cys containing peptides but the requirement of a Cys residue at the ligation junction can limit its use. Here we employ subtiligase variants to eff...
Wedi'i Gadw mewn:
| Cyhoeddwyd yn: | Nat Methods |
|---|---|
| Prif Awduron: | , , , , , , , |
| Fformat: | Artigo |
| Iaith: | Inglês |
| Cyhoeddwyd: |
2016
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| Pynciau: | |
| Mynediad Ar-lein: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5088058/ https://ncbi.nlm.nih.gov/pubmed/27669326 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4004 |
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