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Expressed protein ligation: A general method for protein engineering

A protein semisynthesis method—expressed protein ligation—is described that involves the chemoselective addition of a peptide to a recombinant protein. This method was used to ligate a phosphotyrosine peptide to the C terminus of the protein tyrosine kinase C-terminal Src kinase (Csk). By intercepti...

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Detalles Bibliográficos
Main Authors: Muir, Tom W., Sondhi, Dolan, Cole, Philip A.
Formato: Artigo
Idioma:Inglês
Publicado: The National Academy of Sciences 1998
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC22605/
https://ncbi.nlm.nih.gov/pubmed/9618476
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