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Methods and Applications of Expressed Protein Ligation

Expressed protein ligation is a method of protein semisynthesis and typically involves the reaction of recombinant protein C-terminal thioesters with N-cysteine containing synthetic peptides in a chemoselective ligation. The recombinant protein C-terminal thioesters are produced by exploiting the ac...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Wang, Zhipeng A., Cole, Philip A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7670219/
https://ncbi.nlm.nih.gov/pubmed/32144660
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0434-2_1
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