Enzyme-catalyzed expressed protein ligation

Expressed protein ligation is a valuable method for protein semisynthesis that involves the reaction of recombinant protein C-terminal thioesters with N-Cys containing peptides but the requirement of a Cys residue at the ligation junction can limit its use. Here we employ subtiligase variants to eff...

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Udgivet i:Nat Methods
Main Authors: Henager, Samuel H., Chu, Nam, Chen, Zan, Bolduc, David, Dempsey, Daniel R., Hwang, Yousang, Wells, James, Cole, Philip A.
Format: Artigo
Sprog:Inglês
Udgivet: 2016
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5088058/
https://ncbi.nlm.nih.gov/pubmed/27669326
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4004
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