Specificity of substrate recognition by the EcoRI restriction endonuclease.

The substrate specificity of the EcoRI restriction endonuclease can be varied in vitro by changing the pH and the ionic environment of the reaction. Phosphodiester bond cleavage occurs at a DNA hexanucleotide sequence d(N-G-A-A-T-T-C-N)/d(N-C-T-T-A-A-G-N) when the ionic strength is high, 100 mM Tris...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Polisky, B, Greene, P, Garfin, D E, McCarthy, B J, Goodman, H M, Boyer, H W
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1975
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC432981/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/242001/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.72.9.3310
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