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Specificity of substrate recognition by the EcoRI restriction endonuclease.

The substrate specificity of the EcoRI restriction endonuclease can be varied in vitro by changing the pH and the ionic environment of the reaction. Phosphodiester bond cleavage occurs at a DNA hexanucleotide sequence d(N-G-A-A-T-T-C-N)/d(N-C-T-T-A-A-G-N) when the ionic strength is high, 100 mM Tris...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Polisky, B, Greene, P, Garfin, D E, McCarthy, B J, Goodman, H M, Boyer, H W
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1975
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC432981/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/242001/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.72.9.3310
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