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Specificity of substrate recognition by the EcoRI restriction endonuclease.

The substrate specificity of the EcoRI restriction endonuclease can be varied in vitro by changing the pH and the ionic environment of the reaction. Phosphodiester bond cleavage occurs at a DNA hexanucleotide sequence d(N-G-A-A-T-T-C-N)/d(N-C-T-T-A-A-G-N) when the ionic strength is high, 100 mM Tris...

詳細記述

保存先:
書誌詳細
主要な著者: Polisky, B, Greene, P, Garfin, D E, McCarthy, B J, Goodman, H M, Boyer, H W
フォーマット: Artigo
言語:Inglês
出版事項: 1975
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC432981/
https://ncbi.nlm.nih.gov/pubmed/242001
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