Specificity of substrate recognition by the EcoRI restriction endonuclease.

The substrate specificity of the EcoRI restriction endonuclease can be varied in vitro by changing the pH and the ionic environment of the reaction. Phosphodiester bond cleavage occurs at a DNA hexanucleotide sequence d(N-G-A-A-T-T-C-N)/d(N-C-T-T-A-A-G-N) when the ionic strength is high, 100 mM Tris...

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Dades bibliogràfiques

Publicat a:	Proc Natl Acad Sci U S A
Autors principals:	Polisky, B, Greene, P, Garfin, D E, McCarthy, B J, Goodman, H M, Boyer, H W
Format:	Artigo
Idioma:	Inglês
Publicat:	National Academy of Sciences 1975
Matèries:	Research Article
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC432981/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/242001/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.72.9.3310
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