Determinants of EcoRI endonuclease sequence discrimination.

The arginine at position 200 of EcoRI endonuclease is thought to make two hydrogen bonds to the guanine of the sequence GAATTC and thus be an important determinant of sequence discrimination. Arg-200 was replaced by each of the other 19 naturally occurring amino acids, and the mutant endonucleases w...

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Main Authors: Needels, M C, Fried, S R, Love, R, Rosenberg, J M, Boyer, H W, Greene, P J
Format: Artigo
Jezik:Inglês
Izdano: 1989
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC287181/
https://ncbi.nlm.nih.gov/pubmed/2657723
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