Determinants of EcoRI endonuclease sequence discrimination.

The arginine at position 200 of EcoRI endonuclease is thought to make two hydrogen bonds to the guanine of the sequence GAATTC and thus be an important determinant of sequence discrimination. Arg-200 was replaced by each of the other 19 naturally occurring amino acids, and the mutant endonucleases w...

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Библиографические подробности

Опубликовано в::	Proc Natl Acad Sci U S A
Главные авторы:	Needels, M C, Fried, S R, Love, R, Rosenberg, J M, Boyer, H W, Greene, P J
Формат:	Artigo
Язык:	Inglês
Опубликовано:	National Academy of Sciences 1989
Предметы:	Research Article
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC287181/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2657723/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.10.3579
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