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Determinants of EcoRI endonuclease sequence discrimination.
The arginine at position 200 of EcoRI endonuclease is thought to make two hydrogen bonds to the guanine of the sequence GAATTC and thus be an important determinant of sequence discrimination. Arg-200 was replaced by each of the other 19 naturally occurring amino acids, and the mutant endonucleases w...
Enregistré dans:
| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1989
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC287181/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2657723/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.10.3579 |
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