Specificity of substrate recognition by the EcoRI restriction endonuclease.

The substrate specificity of the EcoRI restriction endonuclease can be varied in vitro by changing the pH and the ionic environment of the reaction. Phosphodiester bond cleavage occurs at a DNA hexanucleotide sequence d(N-G-A-A-T-T-C-N)/d(N-C-T-T-A-A-G-N) when the ionic strength is high, 100 mM Tris...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Polisky, B, Greene, P, Garfin, D E, McCarthy, B J, Goodman, H M, Boyer, H W
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1975
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC432981/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/242001/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.72.9.3310
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