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A fluorescence-based high throughput assay for the determination of small molecule–human serum albumin protein binding

Herein, we describe the development of a fluorescence-based high throughput assay to determine the small molecule binding towards human serum albumin (HSA). This innovative competition assay is based on the use of a novel fluorescent small molecule Red Mega 500 with unique spectroscopic and binding...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Päätekijät: McCallum, Megan M., Pawlak, Alan J., Shadrick, William R., Simeonov, Anton, Jadhav, Ajit, Yasgar, Adam, Maloney, David J., Arnold, Leggy A.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2014
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3959589/
https://ncbi.nlm.nih.gov/pubmed/24390461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-013-7560-3
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