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Re-engineering the polymerase domain of Klenow fragment and evaluation of overproduction and purification strategies.

We describe experiments to produce large quantities of the polymerase domain of E. coli DNA polymerase I for biochemical and biophysical studies. The polymerase domain derivative used in previous studies was insoluble when overproduced and tended to aggregate during purification. These problems were...

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Bibliografiske detaljer
Main Authors: Derbyshire, V, Astatke, M, Joyce, C M
Format: Artigo
Sprog:Inglês
Udgivet: 1993
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC310583/
https://ncbi.nlm.nih.gov/pubmed/8265361
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