Re-engineering the polymerase domain of Klenow fragment and evaluation of overproduction and purification strategies.
We describe experiments to produce large quantities of the polymerase domain of E. coli DNA polymerase I for biochemical and biophysical studies. The polymerase domain derivative used in previous studies was insoluble when overproduced and tended to aggregate during purification. These problems were...
Guardado en:
| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1993
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| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310583/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8265361/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.23.5439 |
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