載入...
Re-engineering the polymerase domain of Klenow fragment and evaluation of overproduction and purification strategies.
We describe experiments to produce large quantities of the polymerase domain of E. coli DNA polymerase I for biochemical and biophysical studies. The polymerase domain derivative used in previous studies was insoluble when overproduced and tended to aggregate during purification. These problems were...
Na minha lista:
| Main Authors: | , , |
|---|---|
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
1993
|
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC310583/ https://ncbi.nlm.nih.gov/pubmed/8265361 |
| 標簽: |
添加標簽
沒有標簽, 成為第一個標記此記錄!
|