Continuous microspectrophotometric measurement of DNA polymerase activity: application to the Klenow fragment of Escherichia coli DNA polymerase I and human immunodeficiency virus type 1 reverse transcriptase.

Progress of DNA- and/or RNA-directed DNA polymerization reactions can be measured continuously using circular dichroism (CD) or ultraviolet (UV) spectroscopy. In the presence of the Klenow fragment of Escherichia coli DNA polymerase I, a CD change of -0.27 +/- 0.06 millidegree at 248 nm and a UV cha...

ver descrição completa

Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Baillon, J G, Nashed, N T, Sayer, J M, Jerina, D M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50945/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1704125/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.3.1014
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!