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Re-engineering the polymerase domain of Klenow fragment and evaluation of overproduction and purification strategies.

We describe experiments to produce large quantities of the polymerase domain of E. coli DNA polymerase I for biochemical and biophysical studies. The polymerase domain derivative used in previous studies was insoluble when overproduced and tended to aggregate during purification. These problems were...

詳細記述

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書誌詳細
主要な著者: Derbyshire, V, Astatke, M, Joyce, C M
フォーマット: Artigo
言語:Inglês
出版事項: 1993
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC310583/
https://ncbi.nlm.nih.gov/pubmed/8265361
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