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Re-engineering the polymerase domain of Klenow fragment and evaluation of overproduction and purification strategies.
We describe experiments to produce large quantities of the polymerase domain of E. coli DNA polymerase I for biochemical and biophysical studies. The polymerase domain derivative used in previous studies was insoluble when overproduced and tended to aggregate during purification. These problems were...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1993
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC310583/ https://ncbi.nlm.nih.gov/pubmed/8265361 |
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