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Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.

Using currently available gene fusion techniques, we have constructed plasmids that direct the overproduction of the carboxyl-terminal two-thirds of DNA polymerase I, corresponding to the proteolytically derived "Klenow fragment." We have obtained overproduction amounting to several percen...

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Bibliografske podrobnosti
Main Authors: Joyce, C M, Grindley, N D
Format: Artigo
Jezik:Inglês
Izdano: 1983
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC393703/
https://ncbi.nlm.nih.gov/pubmed/6340110
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