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Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.

Using currently available gene fusion techniques, we have constructed plasmids that direct the overproduction of the carboxyl-terminal two-thirds of DNA polymerase I, corresponding to the proteolytically derived "Klenow fragment." We have obtained overproduction amounting to several percent of the c...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Joyce, C M, Grindley, N D
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1983
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393703/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6340110/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.7.1830
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