Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.

Using currently available gene fusion techniques, we have constructed plasmids that direct the overproduction of the carboxyl-terminal two-thirds of DNA polymerase I, corresponding to the proteolytically derived "Klenow fragment." We have obtained overproduction amounting to several percent of the c...

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Detaylı Bibliyografya

Yayımlandı:	Proc Natl Acad Sci U S A
Asıl Yazarlar:	Joyce, C M, Grindley, N D
Materyal Türü:	Artigo
Dil:	Inglês
Baskı/Yayın Bilgisi:	National Academy of Sciences 1983
Konular:	Research Article
Online Erişim:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393703/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6340110/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.7.1830
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