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Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases

Gene targeting by homologous recombination in embryonic stem cells is extensively used to generate specific mouse mutants. However, most mammalian species lack tools for targeted gene manipulation. Since double-strand breaks strongly increase the rate of homologous recombination at genomic loci, we...

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Detalles Bibliográficos
Main Authors: Meyer, Melanie, de Angelis, Martin Hrabé, Wurst, Wolfgang, Kühn, Ralf
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 2010
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2930558/
https://ncbi.nlm.nih.gov/pubmed/20686113
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1009424107
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