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Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases

Gene targeting by homologous recombination in embryonic stem cells is extensively used to generate specific mouse mutants. However, most mammalian species lack tools for targeted gene manipulation. Since double-strand breaks strongly increase the rate of homologous recombination at genomic loci, we...

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Bibliografische gegevens
Hoofdauteurs: Meyer, Melanie, de Angelis, Martin Hrabé, Wurst, Wolfgang, Kühn, Ralf
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: National Academy of Sciences 2010
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2930558/
https://ncbi.nlm.nih.gov/pubmed/20686113
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1009424107
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