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Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases

Gene targeting by homologous recombination in embryonic stem cells is extensively used to generate specific mouse mutants. However, most mammalian species lack tools for targeted gene manipulation. Since double-strand breaks strongly increase the rate of homologous recombination at genomic loci, we...

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Bibliografiske detaljer
Main Authors: Meyer, Melanie, de Angelis, Martin Hrabé, Wurst, Wolfgang, Kühn, Ralf
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2930558/
https://ncbi.nlm.nih.gov/pubmed/20686113
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1009424107
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