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Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases
Gene targeting by homologous recombination in embryonic stem cells is extensively used to generate specific mouse mutants. However, most mammalian species lack tools for targeted gene manipulation. Since double-strand breaks strongly increase the rate of homologous recombination at genomic loci, we...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2930558/ https://ncbi.nlm.nih.gov/pubmed/20686113 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1009424107 |
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