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Engineered Zinc Finger Nuclease–Mediated Homologous Recombination of the Human Rhodopsin Gene

PURPOSE. Novel zinc finger nucleases (ZFNs) were designed to target the human rhodopsin gene and induce homologous recombination of a donor DNA fragment. METHODS. Three-finger zinc finger nucleases were designed based on previously published guidelines. To assay for ZFN specificity, the authors gene...

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Detalhes bibliográficos
Main Authors: Greenwald, David L., Cashman, Siobhan M., Kumar-Singh, Rajendra
Formato: Artigo
Idioma:Inglês
Publicado em: Association for Research in Vision and Ophthalmology, Inc. 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3055761/
https://ncbi.nlm.nih.gov/pubmed/20671268
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1167/iovs.10-5781
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