Efficient Knockin Mouse Generation by ssDNA Oligonucleotides and Zinc-Finger Nuclease Assisted Homologous Recombination in Zygotes

The generation of specific mutant animal models is critical for functional analysis of human genes. The conventional gene targeting approach in embryonic stem cells (ESCs) by homologous recombination is however laborious, slow, expensive, and limited to species with functional ESCs. It is therefore...

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Библиографические подробности
Главные авторы: Shen, Bin, Zhang, Xin, Du, Yinan, Wang, Jianying, Gong, Jun, Zhang, Xiaodong, Tate, Peri H., Li, Hongliang, Huang, Xingxu, Zhang, Wensheng
Формат: Artigo
Язык:Inglês
Опубликовано: Public Library of Science 2013
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Research Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3805579/
https://ncbi.nlm.nih.gov/pubmed/24167580
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0077696
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