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High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases
Zinc-finger nucleases (ZFNs) have enabled highly efficient gene targeting in multiple cell types and organisms. Here we describe methods for using simple ssDNA oligonucleotides in tandem with ZFNs to efficiently produce human cell lines with three distinct genetic outcomes: (i) targeted point mutati...
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| Autors principals: | , , , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2011
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3617923/ https://ncbi.nlm.nih.gov/pubmed/21765410 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1653 |
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