High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs

Targeted endonucleases including zinc finger nucleases (ZFNs) and clustered regularly interspaced short palindromic repeats (CRISPRs)/Cas9 are increasingly being used for genome editing in higher species. We therefore devised a broadly applicable and versatile method for increasing editing efficienc...

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Detalhes bibliográficos
Main Authors: Duda, Katarzyna, Lonowski, Lindsey A., Kofoed-Nielsen, Michael, Ibarra, Adriana, Delay, Catherine M., Kang, Qiaohua, Yang, Zhang, Pruett-Miller, Shondra M., Bennett, Eric P., Wandall, Hans H., Davis, Gregory D., Hansen, Steen H., Frödin, Morten
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2014
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4041425/
https://ncbi.nlm.nih.gov/pubmed/24753413
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku251
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