Genome editing using FACS enrichment of nuclease expressing cells and Indel Detection by Amplicon Analysis (IDAA)

This protocol describes methods for increasing and evaluating the efficiency of genome editing based on CRISPR/Cas9, TALENs or ZFNs. First, Indel Detection by Amplicon Analysis (IDAA) determines the size and frequency of insertions and deletions elicited by nucleases in cells, tissues or embryos thr...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Lonowski, Lindsey A., Narimatsu, Yoshiki, Riaz, Anjum, Delay, Catherine E., Yang, Zhang, Niola, Francesco, Duda, Katarzyna, Ober, Elke, Clausen, Henrik, Wandall, Hans H., Hansen, Steen H., Bennett, Eric P., Frödin, Morten
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7250141/
https://ncbi.nlm.nih.gov/pubmed/28207001
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.165
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