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Digital droplet PCR and IDAA for the detection of CRISPR indel edits in the malaria species Anopheles stephensi

CRISPR/Cas9 technology is a powerful tool for the design of gene-drive systems to control and/or modify mosquito vector populations; however, CRISPR/Cas9-mediated nonhomologous end joining mutations can have an important impact on generating alleles resistant to the drive and thus on drive efficienc...

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Detalhes bibliográficos
Publicado no:Biotechniques
Main Authors: Carballar-Lejarazú, Rebeca, Kelsey, Adam, Pham, Thai Binh, Bennett, Eric P, James, Anthony A
Formato: Artigo
Idioma:Inglês
Publicado em: Future Science Ltd 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7177198/
https://ncbi.nlm.nih.gov/pubmed/32040336
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/btn-2019-0103
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