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Target-dependent nickase activities of CRISPR-Cas nucleases Cpf1 and Cas9
Clustered regularly interspaced short palindromic repeat (CRISPR) machineries are prokaryotic immune systems that have been adapted as versatile gene editing and manipulation tools. We found that CRISPR-Cas nucleases Cpf1 (also known as Cas12a) and Cas9 exhibit differential guide RNA sequence requir...
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| Publicado no: | Nat Microbiol |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6512873/ https://ncbi.nlm.nih.gov/pubmed/30833733 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41564-019-0382-0 |
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