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Staphylococcus aureus Cas9 is a multiple-turnover enzyme

Cas9 nuclease is the key effector of type II CRISPR adaptive immune systems found in bacteria. The nuclease can be programmed by a single guide RNA (sgRNA) to cleave DNA in a sequence-specific manner. This property has led to its widespread adoption as a genome editing tool in research laboratories...

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Detalhes bibliográficos
Publicado no:RNA
Main Authors: Yourik, Paul, Fuchs, Ryan T., Mabuchi, Megumu, Curcuru, Jennifer L., Robb, G. Brett
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6298560/
https://ncbi.nlm.nih.gov/pubmed/30348755
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.067355.118
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