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Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci

In order to elucidate the functional organization of the genome, it is vital to directly visualize the interactions between genomic elements in living cells. For this purpose, we engineered the Cas9 protein from Staphylococcus aureus (SaCas9) for the imaging of endogenous genomic loci, which showed...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Main Authors: Chen, Baohui, Hu, Jeffrey, Almeida, Ricardo, Liu, Harrison, Balakrishnan, Sanjeev, Covill-Cooke, Christian, Lim, Wendell A., Huang, Bo
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2016
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4856973/
https://ncbi.nlm.nih.gov/pubmed/26740581
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv1533
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