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Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9

Imaging chromatin dynamics is crucial to understand genome organization and its role in transcriptional regulation. Recently, the RNA-guidable feature of CRISPR-Cas9 has been utilized for imaging of chromatin within live cells. However, these methods are mostly applicable to highly repetitive region...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Qin, Peiwu, Parlak, Mahmut, Kuscu, Cem, Bandaria, Jigar, Mir, Mustafa, Szlachta, Karol, Singh, Ritambhara, Darzacq, Xavier, Yildiz, Ahmet, Adli, Mazhar
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5424063/
https://ncbi.nlm.nih.gov/pubmed/28290446
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms14725
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