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CRISPR-Cas9 enables conditional mutagenesis of challenging loci

The International Knockout Mouse Consortium (IKMC) has produced a genome-wide collection of 15,000 isogenic targeting vectors for conditional mutagenesis in C57BL/6N mice. Although most of the vectors have been used successfully in murine embryonic stem (ES) cells, there remain a set of nearly two t...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Schick, Joel A., Seisenberger, Claudia, Beig, Joachim, Bürger, Antje, Iyer, Vivek, Maier, Viola, Perera, Sajith, Rosen, Barry, Skarnes, William C., Wurst, Wolfgang
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5007477/
https://ncbi.nlm.nih.gov/pubmed/27580957
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep32326
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