Rapid generation of drug-resistance alleles at endogenous loci using CRISPR-Cas9 indel mutagenesis

Genetic alterations conferring resistance to the effects of chemical inhibitors are valuable tools for validating on-target effects in cells. Unfortunately, for many therapeutic targets such alleles are not available. To address this issue, we evaluated whether CRISPR-Cas9-mediated insertion/deletio...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Ipsaro, Jonathan J., Shen, Chen, Arai, Eri, Xu, Yali, Kinney, Justin B., Joshua-Tor, Leemor, Vakoc, Christopher R., Shi, Junwei
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5322889/
https://ncbi.nlm.nih.gov/pubmed/28231254
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0172177
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