CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos

CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in Drosophila. This can facilitate live imaging of nascent transcript...

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Bibliographic Details
Published in:STAR Protoc
Main Authors: Hoppe, Caroline, Ashe, Hilary L.
Format: Artigo
Language:Inglês
Published: Elsevier 2021
Subjects:
Protocol
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7988240/
https://ncbi.nlm.nih.gov/pubmed/33786461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100380
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