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CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos
CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in Drosophila. This can facilitate live imaging of nascent transcript...
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| Publicado en: | STAR Protoc |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7988240/ https://ncbi.nlm.nih.gov/pubmed/33786461 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100380 |
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