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Development of a Cas12a-Based Genome Editing Tool for Moderate Thermophiles

The ability of CRISPR-Cas12a nucleases to function reliably in a wide range of species has been key to their rapid adoption as genome engineering tools. However, so far, Cas12a nucleases have been limited for use in organisms with growth temperatures up to 37 °C. Here, we biochemically characterize...

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Foilsithe in:CRISPR J
Main Authors: Mohanraju, Prarthana, Mougiakos, Ioannis, Albers, Justin, Mabuchi, Megumu, Fuchs, Ryan T., Curcuru, Jennifer L., van Kranenburg, Richard, Robb, G. Brett, van der Oost, John
Formáid: Artigo
Teanga:Inglês
Foilsithe: Mary Ann Liebert, Inc., publishers 2021
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7898400/
https://ncbi.nlm.nih.gov/pubmed/33538626
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/crispr.2020.0086
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