Characterizing a thermostable Cas9 for bacterial genome editing and silencing

CRISPR-Cas9-based genome engineering tools have revolutionized fundamental research and biotechnological exploitation of both eukaryotes and prokaryotes. However, the mesophilic nature of the established Cas9 systems does not allow for applications that require enhanced stability, including engineer...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Mougiakos, Ioannis, Mohanraju, Prarthana, Bosma, Elleke F., Vrouwe, Valentijn, Finger Bou, Max, Naduthodi, Mihris I. S., Gussak, Alex, Brinkman, Rudolf B. L., van Kranenburg, Richard, van der Oost, John
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5698299/
https://ncbi.nlm.nih.gov/pubmed/29162801
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-01591-4
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