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Heterologous Expression and Purification of CRISPR-Cas12a/Cpf1
This protocol provides step by step instructions (Figure 1) for heterologous expression of Francisella novicida Cas12a (previously known as Cpf1) in Escherichia coli. It additionally includes a protocol for high-purity purification and briefly describes how activity assays can be performed. These pr...
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| Publié dans: | Bio Protoc |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Bio-Protocol
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8275269/ https://ncbi.nlm.nih.gov/pubmed/34286046 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2842 |
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