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Introducing gene deletions by mouse zygote electroporation of Cas12a/Cpf1
CRISPR-associated (Cas) nucleases are established tools for engineering of animal genomes. These programmable RNA-guided nucleases have been introduced into zygotes using expression vectors, mRNA, or directly as ribonucleoprotein (RNP) complexes by different delivery methods. Whereas microinjection...
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| Veröffentlicht in: | Transgenic Res |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Springer International Publishing
2019
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6848045/ https://ncbi.nlm.nih.gov/pubmed/31482512 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11248-019-00168-9 |
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