Characterizing a thermostable Cas9 for bacterial genome editing and silencing

CRISPR-Cas9-based genome engineering tools have revolutionized fundamental research and biotechnological exploitation of both eukaryotes and prokaryotes. However, the mesophilic nature of the established Cas9 systems does not allow for applications that require enhanced stability, including engineer...

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Udgivet i:Nat Commun
Main Authors: Mougiakos, Ioannis, Mohanraju, Prarthana, Bosma, Elleke F., Vrouwe, Valentijn, Finger Bou, Max, Naduthodi, Mihris I. S., Gussak, Alex, Brinkman, Rudolf B. L., van Kranenburg, Richard, van der Oost, John
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2017
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5698299/
https://ncbi.nlm.nih.gov/pubmed/29162801
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-01591-4
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