A Tryptophan ‘Gate’ in the CRISPR-Cas3 Nuclease Controls ssDNA Entry into the Nuclease Site, That When Removed Results in Nuclease Hyperactivity

Cas3 is a ssDNA-targeting nuclease-helicase essential for class 1 prokaryotic CRISPR immunity systems, which has been utilized for genome editing in human cells. Cas3-DNA crystal structures show that ssDNA follows a pathway from helicase domains into a HD-nuclease active site, requiring protein conf...

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Detalhes bibliográficos
Publicado no:Int J Mol Sci
Main Authors: He, Liu, Matošević, Zoe Jelić, Mitić, Damjan, Markulin, Dora, Killelea, Tom, Matković, Marija, Bertoša, Branimir, Ivančić-Baće, Ivana, Bolt, Edward L.
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2021
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8001533/
https://ncbi.nlm.nih.gov/pubmed/33799639
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms22062848
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