CRISPR–Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5′ ssDNA exonucleases

Prokaryotic adaptive immunity is established against mobile genetic elements (MGEs) by ‘naïve adaptation’ when DNA fragments from a newly encountered MGE are integrated into CRISPR–Cas systems. In Escherichia coli, DNA integration catalyzed by Cas1–Cas2 integrase is well understood in mechanistic an...

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Publicat a:Nucleic Acids Res
Autors principals: Radovčić, Marin, Killelea, Tom, Savitskaya, Ekaterina, Wettstein, Lukas, Bolt, Edward L, Ivančić-Baće, Ivana
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2018
Matèries:
Genome Integrity, Repair and Replication
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6212769/
https://ncbi.nlm.nih.gov/pubmed/30189098
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky799
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