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Top-down Proteomics of Endogenous Membrane Proteins Enabled by Cloud Point Enrichment and Multidimensional Liquid Chromatography-Mass Spectrometry

Although top-down proteomics has emerged as a powerful strategy to characterize proteins in biological systems, the analysis of endogenous membrane proteins remains challenging due to their low solubility, low abundance, and the complexity of the membrane sub-proteome. Here, we report a simple but e...

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Dettagli Bibliografici
Pubblicato in:Anal Chem
Autori principali: Brown, Kyle A., Tucholski, Trisha, Alpert, Andrew J., Eken, Christian, Wesemann, Lucas, Kyrvasilis, Andreas, Jin, Song, Ge, Ying
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7968110/
https://ncbi.nlm.nih.gov/pubmed/33231430
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.0c02533
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