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Top-down Proteomics of Endogenous Membrane Proteins Enabled by Cloud Point Enrichment and Multidimensional Liquid Chromatography-Mass Spectrometry
Although top-down proteomics has emerged as a powerful strategy to characterize proteins in biological systems, the analysis of endogenous membrane proteins remains challenging due to their low solubility, low abundance, and the complexity of the membrane sub-proteome. Here, we report a simple but e...
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| Publié dans: | Anal Chem |
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| Auteurs principaux: | , , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2020
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7968110/ https://ncbi.nlm.nih.gov/pubmed/33231430 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.0c02533 |
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