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An optimized CRISPR/Cas9 approach for precise genome editing in neurons
The efficient knock-in of large DNA fragments to label endogenous proteins remains especially challenging in non-dividing cells such as neurons. We developed Targeted Knock-In with Two (TKIT) guides as a novel CRISPR/Cas9 based approach for efficient, and precise, genomic knock-in. Through targeting...
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| Publié dans: | eLife |
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| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
eLife Sciences Publications, Ltd
2021
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7946428/ https://ncbi.nlm.nih.gov/pubmed/33689678 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.65202 |
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