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Precise Genome Editing of Drosophila with CRISPR RNA-Guided Cas9

The readily programmable CRISPR/Cas9 system is transforming genome engineering. We and others have adapted the S. pyogenes CRISPR/Cas9 system to precisely engineer the Drosophila genome and demonstrated that these modifications are efficiently transmitted through the germline. Here we provide a deta...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Methods Mol Biol
Main Authors: Gratz, Scott J., Harrison, Melissa M., Wildonger, Jill, O’Connor-Giles, Kate M.
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4467458/
https://ncbi.nlm.nih.gov/pubmed/25981484
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2687-9_22
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