Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila

We and others recently demonstrated that the readily programmable CRISPR/Cas9 system can be used to edit the Drosophila genome. However, most applications to date have relied on aberrant DNA repair to stochastically generate frameshifting indels and adoption has been limited by a lack of tools for e...

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Detalhes bibliográficos
Main Authors: Gratz, Scott J., Ukken, Fiona P., Rubinstein, C. Dustin, Thiede, Gene, Donohue, Laura K., Cummings, Alexander M., O’Connor-Giles, Kate M.
Formato: Artigo
Idioma:Inglês
Publicado em: Genetics Society of America 2014
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Investigations
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3982687/
https://ncbi.nlm.nih.gov/pubmed/24478335
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.113.160713
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