An optimized CRISPR/Cas9 approach for precise genome editing in neurons

The efficient knock-in of large DNA fragments to label endogenous proteins remains especially challenging in non-dividing cells such as neurons. We developed Targeted Knock-In with Two (TKIT) guides as a novel CRISPR/Cas9 based approach for efficient, and precise, genomic knock-in. Through targeting...

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Detaylı Bibliyografya
Yayımlandı:eLife
Asıl Yazarlar: Fang, Huaqiang, Bygrave, Alexei M, Roth, Richard H, Johnson, Richard C, Huganir, Richard L
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: eLife Sciences Publications, Ltd 2021
Konular:
Neuroscience
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC7946428/
https://ncbi.nlm.nih.gov/pubmed/33689678
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.65202
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